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WebMichaelis constants were determined for the two substrates at various pH values. It was found that the affinity of the enzyme for ABTS decreases with increasing pH, and that … WebThe sample or antioxidant standard (injection volume 40 μl), the ABTS •+ solution (injection volume 20 μl) and carrier solution (0.1 M sodium phosphate, pH 7.4) are aspirated … in which in a sentence comma
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WebMar 18, 2024 · DPPH • is commercially available, but ABTS •+ must be produced from the oxidation of ABTS with chemical reagents such as K 2 S 2 O 8, MnO 2, etc. ABTS •+ is soluble in aqueous and in alcoholic media (λ max 734 nm), while DPPH • is soluble in different organic solvents (λ max 517 nm, in ethanol). Web15 hours ago · In this work, the changes in chemical composition (including pH, titratable acid, total and reducing sugar, total soluble solids, protein content, total phenols, and organic acids) and preliminary in vitro bioactivities (including ABTS radical scavenging activity, DPPH radical scavenging activity, α-amylase, and α-glucosidase inhibition) of ... WebFeb 26, 2024 · They found that ABTS is a better choice than DPPH and more sensitive than DPPH. The ABTS method has the extra flexibility in that it can be used at different pH levels, unlike DPPH, which is sensitive to acidic pH as reported by Pękal and Pyrzynska [ 24] and thus is useful when studying the effect of pH on antioxidant activity of various compounds. in which indian city is the taj mahal