Hepg2 crispr

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August undefined, 2024

WebClustered regularly interspaced short palindromic repeats (CRISPR)-associated protein 9 (Cas9) is a precise genome manipulating technology that can be programmed to induce double-strand break (DSB) in the … WebPurpose: The gene editing technology in CRISPR/Cas9 system was used to construct the Traf3 knockout HepG2 cell line to explore the role of Traf3 in the development of liver …

CRISPR/Cas9-mediated knock-out of AGXT1 in HepG2 cells as a …

Web23 apr. 2024 · The gene editing technology in CRISPR/Cas9 system was used to construct the Traf3 knockout HepG2 cell line to explore the role of Traf3 in the development of liver cancer. Methods Five sgRNA sites were designed for the exons of Traf3. WebCXC chemokine receptor 4 (CXCR4) is associated with poor clinical outcomes and decreased survival in hepatocellular carcinoma (HCC). In the present study, we targeted … sbm wins background

Pooled In Vitro and In Vivo CRISPR-Cas9 Screening …

Web4 okt. 2016 · Rapid and cost-effective approaches for assessing the toxicological properties of chemicals are needed. We used CRISPR-Cas9 functional genomic screening to … WebHepG2 cell line is closer to human liver tissue both in terms of morphology and functions. Therefore, this cell line is often used in drug metabolism and liver toxicity studies. The combination of liver cell line and CRISPR/Cas9 helps to study medical problems such as hepatitis, genetic diseases and cancer WebI am culturing HepG2 cells and using lentivirus to knock down the genes. After the knockdown, the cells are screened with puromycin (1ug/mL). I now have a problem with many black dots appearing ... sbm wifi

Successful delivery of large-size CRISPR/Cas9 vectors in …

CIMB Free Full-Text Validation Study to Determine the Accuracy …

WebNational Center for Biotechnology Information Web23 apr. 2024 · The gene editing technology in CRISPR/Cas9 system was used to construct the Traf3 knockout HepG2 cell line to explore the role of Traf3 in the development of liver … sbm wins cornerWeb30 jan. 2024 · In this study, we constructed one HepG2 cell line with a homozygous mutation in the fifth exon of ALDH2 ( ALDH2 -KO1 cell) using the eukaryotic … sbm wins corner poster

"WebCompared with the wild HepG2 cells group, the proliferation and invasion ability of HepG2 cells were enhanced in the Traf3 knockout group. Conclusion. Knockout Traf3 gene by CRISPR/Cas9 system enhanced the proliferation, migration, and invasion of HepG2 cells, and provided an effective tool for studying the function and mechanism of Traf3. " - Hepg2 crispr

Hepg2 crispr

Construction of Traf3 knockout liver cancer cell line using CRISPR…

WebHepG2 CRISPR Transfection Reagent Home Products Cell Line Transfection Kits HepG2 Transfection Kits HepG2 CRISPR Transfection Reagent HepG2 CRISPR Transfection Reagent (BT-000014) * This product is for research use only. Not intended for use in the treatment or diagnosis of disease. Related Products HepG2 Transfection Reagent (BT … WebHepG2 is a human liver cancer cell line that was originally derived from a patient with hepatocellular carcinoma. Cell line is known for the sensitivity to a variety of chemotherapeutic agents, including cisplatin and …

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Web24 nov. 2024 · HEK293FT and HepG2 cells were transfected with Lipofectamine 2000 (Thermo Fisher Scientific) and GenJet HepG2 reagent (SignaGen, SL100489-HEPG2), respectively, according to manufacturer’s ... WebProduct information: Human AGXT CRISPR Knock Out HepG2 Cell Line - Gentaur.com - Product info. Human AGXT CRISPR Knock Out HepG2 Cell Line. Catalog number. K0060256. Price. Please ask . Size. 1x10^6 cells/1.0ml. Cell line type. HepG2. NCBI number. NM_000030. Availability.

WebCYP3A4*1G GG and AA genotype HepG2 cells were established using the clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 (CRISPR/Cas9) single nucleotide polymorphism (SNP) technology and homology-directed repair (HDR) in the CYP3A4*1G GA HepG2 cell line.

Web2 jun. 2015 · To evaluate our anti-HBV CRISPR/Cas9 strategy in a setting of de novo infection, we used HepG2 cells overexpressing the HBV receptor NTCP (Hep-NTCP) 14, which are permissive to infection with... Web6 mrt. 2024 · c, p53-depleted and control HCT116, U2OS or HepG2 cells were assayed for ODC expression. d, Gene expression in p53 +/+ and p53 −/− HCT116 cells was …

Web16 dec. 2014 · To investigate whether cccDNA derived from infectious HBV could be directly targeted for destruction, we used the CRISPR/Cas9 system in HepG2 cells expressing the HBV receptor sodium taurocholate cotransporting polypeptide (NTCP). We tested different HBV-specific guide RNAs and demonstrated that they could inhibit HBV infections up to …

Web29 okt. 2024 · CRISPR-Cas9 genome editing was used to target SPRY2 in HepG2 cells, and the functional consequences of SPRY2 knockout (KO) and overexpression … sbm yellowtailWebCYP3A4*1G GG and AA genotype HepG2 cells were established using the clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 … sbm wood repairWeb2 jun. 2016 · The anti-HBV effect was estimated by the designed CRISPR/Cas system in hepatoma cells of Huh7 and HepG2. The pcHBV1.3 plasmid and sgRNA-Cas9 (pCas9) constructs were co-transfected into Huh7 and HepG2 cells, and cccDNA, replication intermediate, HBcAg, HBsAg, HBeAg secretions of HBV were measured. sbm wins mission and visionWeb14 rijen · Cell-Line Specific CRISPR RNP Electroporation Conditions Using Neon System. … sbm wins logoWebHepg2 Hntcp C4 Cells, supplied by Promega, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, ... Article Title: Inhibition of nonhomologous end joining‐mediated … sbm-150wntWeb7 mei 2024 · HepG2 cells were chosen because they are TGF-β sensitive (Senturk et al., 2010) and have previously been used for CRISPR-Cas9 screens (Xia et al., 2016). … sbm wooden shearsWebThe CRISPR (clustered regularly interspaced short palindromic repeats)/Cas9 genome editing system has been a major technological breakthrough that has brought … sbm wins organizational chart