Cisplatin binding to holliday junction

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August undefined, 2024

Webformation of Holliday junction 1. after strand invasion, the two DNA molecules are connected by crossing DNA strands to form the Holliday junction 2. junction can move along the DNA by repeated DNA melting and formation of base pairs 3. process is called branch migration resolution of Holliday junction WebThe BCDX2 complex also bound to the synthetic Holliday junction, and its af®nity to the Holliday-junction DNA was signi®cantly higher than that of Rad51B (Fig. 4).

Structural Similarities between Escherichia coli Ruva Protein …

WebFour-way DNA intermediates, called Holliday junctions (HJs), can form during meiotic and mitoticrecombination,andtheirremovaliscrucialforchromosomesegregation.Agroupof ubiquitous and highly specialized structure-selective endonucleases catalyze the cleavage of HJs into two disconnected DNA duplexes in a reaction called HJ resolution. WebMay 15, 2007 · This binding mode is specific for the Holliday junction and does not cause unwinding of the DNA helices. Citing Literature Supporting Information Volume 46, Issue … dvd 書き込み方法 windows10

Holliday Junction Binding Activity of the Human Rad51B Protein

WebMar 15, 1997 · The results confirm that RuvC protein binds Holliday junctions as a dimer. Influence of DNA sequence on incision Previously it was shown that Holliday junction cleavage by RuvC occurs at specific sequences, with the consensus defined as 5′- A / T TT ↓G / C -3′. Single base changes within this sequence severely reduce the efficiency of … WebAug 5, 2011 · Holliday junctions are formed during recombination, double-strand break repair, and fork reversal during replication. Resolving Holliday junctions is a critical process for maintaining genomic stability [ 25, 26 ]. These junctions are resolved by a class of structure-specific nucleases: the junction-resolving enzymes. Figure 1 WebPMID: 9535214 Abstract Proteins that can be shown to strongly bind in vitro to the four-way (Holliday) junction DNA include not only the obvious candidates such as enzymes involved in recombination, but also a remarkably diverse group of seemingly unrelated proteins. dvd版 office 2021 professional plus oem版

IJMS Free Full-Text Integration of DNA Damage and Repair with ...

WebOct 5, 2014 · 1. Introduction. Cisplatin (CAS No. 15663-27-1, MF-Cl 2 H 6 N 2 Pt; NCF-119875), cisplatinum, also called cis-diamminedichloroplatinum(II), is a metallic (platinum) coordination compound with a square planar geometry.It is a white or deep yellow to yellow-orange crystalline powder at room temperature. It is slightly soluble in water and soluble … crystal bell actressWebBinding of RuvA to the Holliday Junction109 phosphate atoms across the minor groove in B-DNA. It is tempting to speculate that the arrangement of the two approximately 2-fold- related HhH... dvd版 office professional plus2021

"WebCisplatin is a platinum-based agent whose nephrotoxicity is thought related to the chloride in the cis position. Cisplatin gains entry into tubular cells via uptake by the OCT-2 … " - Cisplatin binding to holliday junction

Cisplatin binding to holliday junction

Structural Similarities between Escherichia coli Ruva Protein …

WebAbstract. In the present paper, the interactions of the origin binding protein (OBP) of herpes simplex virus type 1 (HSV1) with synthetic four-way Holliday junctions (HJs) were … WebThe binding of Holliday junction DNA by endo-exonuclease. Purified endo-exonuclease (5 units) was incubated with 32P-labeled junction molecules, linear ss-DNA (146 nucleotides), and linear ds-DNA ...

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WebFeb 21, 1997 · The E. coli RuvA and RuvB proteins, which are involved in the late stages of recombination and the recombinational repair of damaged DNA, bind to Holliday junctions and promote branch migration. We have used electron microscopy and image analysis to examine RuvA and RuvB bound to model Holliday structures. WebNational Center for Biotechnology Information

WebFrom PDB: 3CRX . Schematic diagrams of the three base-stacking conformational isomers of the Holliday junction. The two stacked conformers differ in which sets of two arms are … WebApr 3, 2007 · The bifunctional binding of the anticancer drug cisplatin to two adjacent nucleobases in DNA is modeled using density functional theory. Previous experimental studies revealed that cisplatin binding to adjacent guanine and adenine is sensitive to nucleobase sequence.

WebCX3 (A) or BCDX2 (B) was incubated with Holliday junction templates, mounted onto carbon-coated copper grids, and rotary shadowcast with tungsten for visualization by EM. Images are shown in ... WebConformational switching of the synthetic Holliday junction (HJ) labeled with a Cy3-Cy5 fluorophore pair. (b) Representative single molecule fluorescence-time traces from our …

WebFeb 1, 2003 · When the synthetic Holliday junction was present along with the half-cruciform and double-stranded oligonucleotides, the Rad51B protein only bound to the synthetic Holliday junction, which...

WebJun 3, 2024 · Wang, W. et al. Holliday junction thermodynamics and structure: coarse-grained simulations and experiments. Sci. Rep. 6, 22863 (2016). crystal bella wigs ottawaWebDNA polymerases fill in 3′ overhangs, whereby the D-loop becomes a Holliday junction. RAD52 functions to anneal the second end of DNA leading to a Holliday junction. BLM migrates the two ends of the Holliday junction in the direction of one another with some filling in by polymerases if necessary. dvd版 microsoft office professional 2019WebAug 27, 2024 · Brogden, A.L.; Hopcroft, N.H.; Searcey, M.; Cardin, C.J. Ligand bridging of the DNA Holliday junction: Molecular recognition of a stacked-X four-way junction by a … crystal belize car rentalWebJul 11, 2000 · Actually, both of the RuvA tetramers, the upper and the lower, simultaneously contact each strand of a DNA duplex arm ( 16 ). By contrast, in complex I, the two … dve20a3200wWebway DNA structure, also called a Holliday junction, which is processed by the RuvABC proteins.2 The RuvA protein initially binds the Holliday junction, and then recruits RuvB protein rings to opposite sides of the junction.3,4 RuvB is a molecular motor that uses the energy derived from ATP binding and hydrolysis to drive unidirectional movement ... crystal belize discount codeWebTo expose two cut sites, the Holliday junction is rotated by 180° to form a sequare planar structure. Resolution of Holliday junction occurs by cutting the DNA strands at the site of cross and re-joining them. Resolution occurs in one of the two ways. This gives rise to two classes of DNA products. crystal bellandurWebSep 15, 2024 · Cisplatin (Cisplatin Injection) may treat, side effects, dosage, drug interactions, warnings, patient labeling, reviews, and related medications including drug … dvd 読み込まない windows11